CDW cell dry weight CETSA cellular thermal shift assay GCMS gas chromatography-mass spectrometry LC-MS liquid chromatography-mass spectrometry LFQ label-free quantitation PHA polyhydroxyalkanoate PHB polyhydroxybutyrate TPP thermal proteome profiling 1 Introduction Cupriavidus necator is a bacterium with broad metabolic capacity that reflects its wide ecological distribution, which includes aquatic and soil environments, as well as industrial and polluted locations.