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Abstract Brassica napus is a globally important allotetraploid oilseed crop with a complex genome and extensive structural diversity that remains incompletely characterized. Here, we present gap-free reference genome assemblies for Zhongshuang 11 (ZS11) and Westar, and assemble six additional accessions. We harmonize gene annotations across 22 genomes and develop BnaScope (https://rgmi.hzau.edu.cn/bnascope), a gene-centric functional genomics platform.
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