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LPS-primed Stx2-induced HUS (LPS-Stx2 HUS) in mice28 was established for the study. C57BL/6 mice (6–10 weeks old, 18–22?g) purchased from the Jackson Laboratory were housed in a pathogen-free animal facility. All experiments using animals and procedures of animal care and handling were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of NIH.
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