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Abstract Detection of postoperative minimal residual disease (MRD) using circulating tumor DNA (ctDNA) accurately predicts cancer recurrence and may even guide adjuvant chemotherapy. Cost-effective and easily implementable assays capable of detecting MRD early (within days after surgery) will drive wider clinical adaptation. We developed a rapid, single-tube multiplex quantitative methylation-specific PCR (mqMSP) assay targeting 10 rigorously screened methylation markers.
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