As mentioned above, computational and methodological issues hindered the utilization of the algorithm developed by Irianto et al. to quantify chromatin condensation in situ. The first problem that needed to be solved was the independent thresholding of the data. To prevent biasing in our analysis and retain comparable datasets, our algorithm takes into consideration the entirety of the dataset to be analyzed and performs only a single thresholding calculation for all the data collectively.
