Biofuels have become important renewable energy alternative to fossil fuels in modern days. The non-food woody lignocellulose biomass drawn more attention to produce biofuel since it is not against the world's food security. Among which, poplar serves as an ideal woody biomass because of its fast growth and inherent secondary cell wall characteristics (Cho et al., 2018). Lignocellulose (secondary cell wall) is mainly composed of lignin, cellulose and hemicellulose.