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1 Introduction Global climate change and the increasing frequency of extreme temperature events pose significant threats to crop production worldwide. High temperatures, particularly during critical growth stages, can adversely affect crop yield and quality, thus threatening food security (Bita and Gerats 2013).
Genotyping-in-thousands by sequencing (GT-seq) GT-seq is a method of next-generation sequencing of multiplexed PCR products and is used to generate genotypes (Campbell et al., 2015). GT-seq enables thousands of individual samples to be sequenced in a single Illumina HiSeq lane, which greatly improves the efficiency of large-scale genomics research, especially for population genetic studies.
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