Abstract Living donor liver transplantation (LDLT) traditionally relies on static cold preservation, which may contribute to ischemia–reperfusion injury associated with abrupt temperature transitions. This study investigated the feasibility of immediate liver transplantation without cold storage using rapid magnetic anastomosis. Eighty-eight adult male Sprague–Dawley rats were randomly divided into two groups: Cold Storage Transplantation Group (CSTG) and Immediate Transplantation Group (ITG).