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Abstract Introduction: Sex-based differences in immune responses to vaccination are well-documented, yet the underlying epigenetic mechanisms remain poorly understood. This study investigates DNA methylation profiles in B cells following hepatitis B virus (HBV) vaccination, with a focus on sex-specific patterns. Methods: Using high-resolution genome-wide methylation analysis, we examined post-vaccination samples from healthy male and female health care workers.
Out of 56 assessed samples, 6 samples failed DNA quality check and were removed from downstream processing. The final analysis included 50 samples—27 in the survived group and 23 in the deceased group—with a total of 662′889 probes each. Baseline subject’s characteristics are summarized in Table 1.
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