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Heterogeneous spatial organization of tumor-infiltrating lymphocytes was associated with immune evasion and tumor evolution in NSCLC (2, 33). Specifically, the presence of TLS has been shown to correlate with prolonged survival in NSCLC and a variety of cancer types (28, 33–36). However, immune rich areas beyond TLS are not well studied. Immune-rich areas differ from TLS in terms of cellular densities, organizations, and spatial distributions (37–39).
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