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Introduction Blebbistatin, a myosin II ATPase inhibitor (Straight et al., 2003), has been used extensively in optical mapping research as an effective excitation–contraction (EC) uncoupling agent (Fedorov et al., 2007). However, recent evidence suggests that blebbistatin might increase arrhythmia susceptibility (Brack et al., 2013; Kappadan et al., 2020).
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