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Cell & Gene Therapy Insights 2022; 8(8), 985–992 DOI: 10.18609/cgti.2022.146 Transient transfection is a challenging and time-sensitive upstream unit operation to scale for suspension HEK293 viral vector production. For many transfection reagents, including polyethyleneimine, careful timing of mixing and hold steps is essential to process performance but challenging to maintain at relevant manufacturing scales.
As a journalist, you can create a free Muck Rack account to customize your profile, list your contact preferences, and upload a portfolio of your best work.