Abstract Lipidomics provides critical insights into disease mechanisms, biomarker discovery, and precision medicine, but conventional LC-MS workflows often require sample volumes that limit their application in minimally invasive studies. Here, we evaluated a nanoflow liquid chromatography workflow coupled with trapped ion mobility spectrometry and time-of-flight mass spectrometry (nanoLC-TIMS-TOF) for positive ion lipid profiling from as little as 1 µL of plasma.
