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To compare the response of Zur, ZntR, RcnR, FrmR and FrmRE64H to Zn(II) and cobalt, Salmonella cells were cultured in minimal media (~4–5?h) supplemented with MNICs of each metal (giving ?10% inhibition of growth), ensuring multiple cell division cycles in the presence of metal (Supplementary Fig. 2). Transcript abundance was visualised by end point reverse-transcriptase PCR and enumerated by quantitative PCR (qPCR; Fig. 2a–e).
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