Abstract Pine and blossom honey have different levels of consumer appeal. To avoid fraud and adulteration, it is important to distinguish between the two types of honey. The identification of honey as blossom and pine remains difficult. In the current study, palynological parameters, phenolics, organic acids, sugars, and minerals were investigated in blossom and pine honey samples to establish an accurate botanical differentiation. Initially, honey samples were tested using palynological analysis.