In the present prospective cohort study, we found that a higher frequency of (lean, fatty and total) fish intake was significantly associated with lower odds of physical pre-frailty after 8 years in older community-dwelling adults in Norway. Moreover, a pattern of consistent high frequency of total fish intake over 21 years was associated with lower odds of pre-frailty. Overall, the main study population was a relatively healthy sample of older residents in Tromsø, Northern Norway.