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Articles by Leon Reubsaet
Special Issue on the 18th Nordic Mass Spectrometry Meeting in Geilo, Norway
EDITORIAL Special Issue on the 18th Nordic Mass Spectrometry Meeting in Geilo, Norway Léon Reubsaet, Corresponding Author Léon Reubsaet Section of Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, Oslo, Norway Correspondence: Léon Reubsaet (leonr@farmasi.uio.no) Jonas Bergquist (jonas.bergquist@kemi.uu.se) Search for more papers by this authorJonas Bergquist, Corresponding Author Jonas Bergquist Analytical Chemistry, Department of Chemistry BMC, Uppsala University,...
Advancements in clinical approaches, analytical methods, and smart sampling for LC-MS‐based protein determination from dried matrix spots
ABC ammonium bicarbonate CMC carboxymethyl cellulose DBS dried blood spot DDA data-dependent acquisition DIA data-independent acquisition DSeS dried serum spots DTT dithiothreitol DVS divinyl sulfone Eu Europium Hb Hemoglobin hCG human chorionic gonadotropin Hct Hematocrit HEMA 2-hydroxyethyl methacrylate HR high resolution IAA iodoacetic acid ICP inductively coupled plasma IMER immobilized-enzyme reactor LC–MS liquid chromatography–mass spectrometry LcrV low calcium response V mAbs...
Smart sampling as the “Spot‐on” Method for LC‐MS protein analysis from dried blood spots
bmAbs biotinylated mAbs DBS dried blood spots HCT hematocrit hCG human chorionic gonadotropin mAb monoclonal antibodies MIPs molecularly imprinted polymers 1 INTRODUCTION The use of paper in the clinical analysis of biologically relevant substances has a long history. Already at the beginning of the 20th century, Ivar Bang showed the possibility of determining glucose from blood deposited on filter paper [1] (Figure 1).
Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry
LC-MS: a versatile and sensitive tool for DBS analysis Mass spectrometry is highly recognized for its sensitivity and selectivity, allowing for low risks of false positives and negatives when operating within the boundaries of a validated method. With MS, the exact mass of the analyte is determined, often followed by a fragmentation of the analyte, and finally mass determination of these fragments.
Pre-lab proteolysis for dried serum spots - a paper-based sampling concept targeting low abundant biomarkers
Pre-lab proteolysis for dried serum spots – a paper-based sampling concept targeting low abundant biomarkers† Paper-based sampling of biological matrices in combination with mass spectrometry has proven to be a promising technique for bottom-up analysis of proteins. However, these techniques have been challenging for determining low abundance proteins (pg mL−1 to low ng mL−1) due to low sampling volumes and adsorption of proteins to the sampling material.
Pre-lab proteolysis for dried serum spots - A paper-based sampling concept targeting low abundant biomarkers
Pre-lab proteolysis for dried serum spots - A paper-based sampling concept targeting low abundant biomarkers Paper-based sampling of biological matrices in combination with mass spectrometry has proven to be a promising technique for analysis of low abundant proteins. However, these techniques have been challenging for determining low abundance proteins (pg/mL- low ng/mL) due to low sampling volumes and adsorption of proteins to the sampling material.
Smart blood spots for whole blood protein analysis
A reactor for whole blood sampling integrated with instant protein digestion in a “lab-on-paper” format is introduced here. The sampling reactor was fabricated on commercial available filter paper using HEMA-VDM polymerization followed by immobilization of trypsin. Immobilization conditions were investigated with respect to temperature, enzyme amount and immobilization time.
Instant on-paper protein digestion during blood spot sampling
A concept integrating sampling and protein digestion is here introduced combining fast and simple fabrication by wax printing on filter paper with trypsin immobilized polymer beads. The paper reactors showed promising results with a high degree of protein digestion within fifty minutes in model protein mixtures as well as in human blood. The model protein mixture was used for evaluation of performance both with and without a reduction and alkylation step.
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