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Previously, we reported the development of long-term-estrogen-deprived (LTED) derivatives from a number of ESR1-positive BC cell lines (including MCF7, HCC1428, T47D, ZR75.1, and SUM44)14,15. In general, estrogen deprivation leads to an initial quiescent population accompanied by cell death and after many weeks to outgrowth of a cell population that then proliferates independently of exogenous estrogen (Supplementary Fig. 1a–d).
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