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Nitric oxide (NO) is a fundamental signaling molecule virtually regulating every critical cellular function, and also a potent mediator of cellular damage in a wide range of conditions mainly via its secondary metabolites peroxynitrite (ONOO-). In this work, we present an o-phenylenediamine (OPD)-locked Si-rhodamine deoxylactam, i.e. deOxy-DALSiR, as a near-infrared fluorescent probe for selective and sensitive detection of NO in living cells and bodies.
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