3. Discussion In this paired culture study, we showed that 2%HPL is a suitable xeno-free supplement substitution for 2%FBS in human donor cornea organ culture. We detected no significant alterations in morphology of the CECs during the 25-day differential culture period. The ECL was significantly lower in 2%HPL vs. 2%FBS. NGS showed differences in gene expression predominantly in the DmE (1644 genes) and less in the stroma (217 genes). CSK markers were not influenced.
