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Abstract Vaccines have played a central role in combating the COVID-19 pandemic, but newly emerging SARS-CoV-2 variants are increasingly evading first-generation vaccine protection. To address this challenge, we designed “single-cycle infection SARS-CoV-2 viruses” (SCVs) that lack essential viral genes, possess distinctive immune-modulatory features, and exhibit an excellent safety profile in the Syrian hamster model.
2.6. Experimental Procedure (Formaldehyde) The principal inactivation procedure followed four sequential steps: First, cells were infected with the titrated virus inoculum. In the second step, infected cultures were treated with formaldehyde. In the third step, adherent cells were harvested at different time points by scraping.
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