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Abstract High-throughput sequencing technologies have greatly advanced our understanding of microbiomes, but resolving microbial communities at species and strain levels remains challenging. Here, we developed and validated a pipeline for designing, multiplexing, and sequencing highly polymorphic taxon-specific long-read amplicons.
Abstract High-throughput sequencing technologies have greatly advanced our understanding of microbiomes, but resolving microbial communities at species and strain levels remains challenging. In this study, we developed and validated a pipeline for designing, multiplexing, and sequencing highly polymorphic taxon-specific amplicons using PacBio circular consensus sequencing.
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