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Introduction Experimental animals exhibit various behaviors, such as ambulation, immobility, rearing, scratching, and grooming. Since behavior reflects the mental and physical condition of an animal, we can estimate it by observing its behavior. Grooming is one of the common behaviors to care for fur, maintain hygiene, and regulate body temperature in experimental animals such as mice, rats, and others (Almeida et al., 2015; Kalueff et al., 2016).
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