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Tissue culture and reagents Cell lines were obtained from ATCC (American Type Culture Collection, Chicago, IL, USA) and used at low passage. Cells were cultured in DMEM (A431, U2OS, HEK293T) or RPMI 1640 (NCI-H520, LUDLU1, CALU-1, NCI-H522, NCI-H1299, A549) supplemented with 10% foetal calf serum (FCS), 2 mmol/L glutamine and 100 U/mL penicillin/streptomycin (all Sigma). Delipidated FCS was prepared as previously described [23].
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