TRAMP-C2 cells acquired from American Type Culture Collection (ATCC) and cultured according to ATCC specifications. The TRAMP-C2 cells were transduced with a customized lentiviral vector carrying tetracycline (syn. doxycycline-; “DOX”) inducible PSMA expression system. Creating a DOX-inducible expression system was performed as follows: First, PSMA was sub-cloned out of an expression plasmid EX-G0050-Lv205 (GeneCopoeia, Inc.) with EcoRI and BamH1 and ligated into the target pLVX-TRE3G vector.
