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Protein dynamics can be studied using hydrogen–deuterium exchange as measured by mass spectrometry or NMR (HDX-MS or HDX-NMR). First demonstrated by Linderstro̷m-Lang, (1) HDX-MS can report on both global and local dynamics in proteins. Compared to NMR, HDX-MS brings higher sensitivity at low protein concentrations, no limitations due to protein size, and detection of multiple coexisting protein conformers.
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