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The waste coffee in the capsule container of nine different Nespresso machines operated for at least one year was sampled (Fig. 1A). In one case (CityZ model), the cup tray was also sampled independently as it does not connect with the capsule container. The high throughput sequencing and analysis of the 16S rRNA gene amplicons from all the machines revealed a significant bacterial diversity, with the total number of identified genera ranging from 35 to 67.
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