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Abstract Engineering robust fermentation hosts remains a barrier to scalable biomanufacturing. In this work we replace canonical mixed-acid fermentation in Escherichia coli with a redox‑balanced reverse β‑oxidation (rBOX) pathway that produces medium‑chain fatty alcohols and is bioenergetically equivalent to ethanol fermentation.
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Genetics and Molecular Biology Mitchell G. Thompson, Matthew R. Incha, Allison N. Pearson, Matthias Schmidt, William A. Sharpless, Christopher B. Eiben, Pablo Cruz-Morales, Jacquelyn M. Blake-Hedges, Yuzhong Liu, Catharine A. Adams, Robert W. Haushalter, Rohith N. Krishna, Patrick Lichtner, Lars M. Blank, Aindrila Mukhopadhyay, Adam M. Deutschbauer, Patrick M. Shih, Jay D.
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