CPA induces cell death, not cell proliferation. (A) Representative images (n = 3) of Ki67 staining in PD5 (72 h) or 8-week-old mouse ovary 1 day (24 h) after injection of PBS or 150 mg/kg CPA. Granulosa (orange) and somatic cells (red) were marked with arrows. Green = Ki67, blue = DAPI. Scale bar, 50 µm. (B) Representative images of TUNEL staining of PD5 CD-1 mouse ovary 1, 3 and 7 days after injection of 150 mg/kg CPA or PBS. Green = apoptotic cells, blue = DAPI. Scale bar, 50 µm.
