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Abstract Protein phosphorylation cascades are essential for cell proliferation. Here, we identify protein arginine methyltransferase 1 (PRMT1) as a key stabilizer of phosphoproteins that is indispensable for hepatocyte proliferation during liver regeneration. PRMT1 is upregulated by epidermal growth factor (EGF) via receptor tyrosine kinase signaling and catalyzes the asymmetric dimethylation (ADMA) modification of SKP1 at the R81 residue.
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