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Abstract While mRNA alternative splicing caused by somatic mutation in SF3B1 is well-characterized, long non-coding RNAs (lncRNAs) impacted by this mutation are completely unknown. Here, we report long-read sequencing of full-length transcripts from cell lines with and without SF3B1 mutation. We present ALLR (Annotation of human lncRNAs using Long-Read sequencing), a computational workflow to identify and quantify lncRNAs, and to annotate their potential roles in impacting cellular functions.
There is much upon which we, as Americans, seem to disagree these days. However, one thing I believe we all agree upon is that our immigration system is failing and needs a complete overhaul. Since its inception, America has been a haven for oppressed people seeking freedom. It has been a land of endless promise for those willing to work hard and follow the rules.
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