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Abstract Plant development is highly plastic, with growth and architecture continuously shaped by environmental conditions. Roots, for instance, adjust their development to local water, nutrient and soil conditions. Despite this plasticity, individual root cell types are generally viewed as largely homogeneous populations that progress along a single developmental trajectory1.
Abstract Synthetic gene circuits are powerful tools for precisely programming gene expression and introducing novel cellular functions. However, their development and application in plants has lagged behind other systems, due mainly to the limited availability of modular genetic parts. We recently developed a CRISPR interference (CRISPRi)-based synthetic gene circuit system for programming gene expression in plants.
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