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CONFLICT OF INTEREST STATEMENT The authors declare no conflict of interest. DATA AVAILABILITY STATEMENT The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions. Supporting Information Filename Description bcp70446-sup-0001-Supporting_Information.docxWord 2007 document , 511.2 KB Table S1.
Abstract Efficient DNA delivery is essential for genetic manipulation of mycobacteria and for dissecting their physiology, pathogenesis, and drug resistance. Although electroporation enables transformation efficiencies exceeding 105 CFU per µg DNA in Mycobacterium smegmatis and Mycobacterium tuberculosis, it remains highly inefficient in many non-tuberculous mycobacteria (NTM), including Mycobacterium abscessus. Here, we discovered that NTM such as M.
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