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ABSTRACT To develop a three-dimensional corneal construct suitable for in vitro studies of disease conditions and therapies. /Methods: In vitro human corneal constructs were created using chemically cross-linked collagen and chondroitin sulfate extracellular matrix and seeded with three human corneal cell types (epithelial, stromal, and endothelial) together with neural cells.
1. Introduction Herpes Simplex Virus serotype 1 (HSV-1) infection is the leading infectious cause of unilateral corneal blindness in developed nations and the third most common indication for patients requiring corneal transplantation worldwide [1,2,3,4,5,6]. A million new and 9,000,000 recurrent cases are reported each year globally [7].
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