The human BTC cell lines SNU245, SNU308, SNU478, SNU869, SNU1079, and SNU1196 were purchased from the Korea Cell Line Bank (KCLB, Seoul, Korea). These cell lines were maintained in RPMI1640 (Invitrogen Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT, US). NIH-3T3 mouse fibroblast cells were purchased from the American Type Culture Collection (ATCC Manassas, VA, USA) and maintained in DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS.
