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Abstract R-loops are a class of non-canonical nucleic acid structures that typically form during transcription, when the nascent RNA hybridizes with the DNA template strand, leaving the DNA coding strand unpaired. Co-transcriptional R-loops are abundant in nature and biologically relevant. Recent research shows that DNA sequence and topology affect R-loops, and yet it remains unclear how these and other factors drive R-loop formation.
Research Article This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.
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