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2. Results 2.1. Effects of Pulse Durations in HBSS Buffer at 20 Hz Live fluorescence microscopy was used to assess the long-term impact of nsPEF exposure by observing (1) the induced cell death in the spheroids 32 h after nsPEF exposure and (2) cells’ ability to re-grow over a five-day period. Spheroids were subjected to 500 unipolar pulses of 2, 4, 6, 8, and 10 ns duration (referred to as Protocol 1), applied at a 20 Hz repetition rate.
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