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NP (99% purity) was purchased from Aladdin, dissolved in methanol to obtain a 1?g·L?1 stock solution and stored at ?20?°C in the dark. Four hundred of three-month-old zebrafish (Danio rerio) were purchased from a local supplier. They were transferred to the laboratory and acclimated for at least one month in flow-through holding tanks supplied with aerated freshwater, kept at 26?±?1?°C with a photoperiod of 14?h:10?h light: dark.
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