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By Dr Patrick Guye, Dr Eva Thoma and Dr Olivier Frey Winter 2018/19 Life operates in three spatial dimensions (3D), as do the patients for whom we are developing therapies and cures. Earlier limitations in cell culture and analysis methods constrained us to 2D cell culture substrates, but the amount of valuable information we can extract from such systems is quite limited, and largely exhausted.
The PGP1, PGP5 and PGP9 hiPSCs were a kind gift from George Church (Harvard University, USA) and can be obtained from Coriell (NJ, USA). The C1 hiPSC was a kind gift from Rudolf Jaenisch (MIT/Whitehead, USA). Cells were cultivated under sterile conditions in mTeSR-1 (Stem Cell Technologies, Vancouver) in a humidified incubator at 37 °C and 5% CO .
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