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MB medium was made similar to the commercial Marine Broth 2216 medium (BD Biosciences, #279110), but in comparison shows no precipitate. Chemicals [g/L]: Peptone 5.0, yeast extract 1.0, ferric acid citrate 0.1, MgCl 6H O 12.6, Na SO 3.24, NaCl 19.45, CaCl 2H O 2.38, KCl 0.55, NaHCO 0.16, Na HPO 2H O 0.01, agar 17.7 (for agar plates only).
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