Recombinant expression in mammalian cells The genes encoding the antibody VH and VL chains are inserted into expression vectors and transferred into suitable cell lines for high-yield protein production.8 The most frequently used cell lines for this purpose in the biotechnology industry include Chinese hamster ovary (CHO) cells, human embryonic kidney (HEK293) cells, and murine myeloma (NS0) cells.8, 78 CHO cells are widely preferred due to their ability to perform human-like...
