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Abstract Recombinant adeno-associated virus (rAAV) holds immense promise for therapeutic applications, but high production costs limit its widespread clinical use, underscoring the urgent need for manufacturing breakthroughs. Here, we establish a modular Mono-Bac system with the p24-NanoLuciferase (Nluc) reporter Bacmid for rAAV production, named mMono-Bac1.0, enhancing manufacturing flexibility, throughput and yield simultaneously.
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