Acknowledgement This work was supported in part by NIH 5UM1HG012077-02. D.K.S. was funded by the UCLA-Caltech Medical Scientist Training Program (NIH NIGMS training grant T32 GM008042). We thank Bernadett Gaál for the feedback on dedicating a study to package comparisons in the scRNA-seq workflow. We thank Tara Chari for providing feedback and assisting with data management. The authors acknowledge the Howard Hughes Medical Institute for funding A.S.B. through the Hanna H. Gray Fellows program.