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P. granatum tree var. ‘Wonderful’ (PG-100-1) trees were grown in the nursery at the Newe-Yaar Center of the Agricultural Research Organization (ARO). We covered young leaves to obtain etiolated leaves and immediately froze them in liquid nitrogen. To remove residual polysaccharides and salts and to purify high-molecular-weight (HMW) genomic DNA, a salt: chloroform-based cleanup was performed following PacBio SampleNet protocols.
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