Discussion The objective of this open rapid publication is stimulation of further research regarding the peptides and parental proteins which we found to be differentially regulated in a population of 50 ALS patients compared to peptides in non-ALS individuals. It is important to understand that the identification of peptides in our study was performed without previous protease digestion in a preparatory one-pot approach and holistically (capturing all peptides up to 20 kDa molecular mass) [13].
