Abstract Background: The use of autologous growth factors, such as those contained in Hypoxia-Preconditioned Serum (HPS) and Platelet-Rich Plasma (PRP), represents a promising strategy to enhance regenerative processes in injured nerves. As an initial in vitro study, we investigated the effects of these secretomes on the neuroblastoma cell line N2a. Methods: HPS and PRP were first compared with normal serum (NS) using a protein microarray to detect neurologically related growth factors.