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This study was undertaken at the ZCC of Tubarão (28°31′12.64″S, 49°01′03.09″W), located in the southern region of Santa Catarina (Fig. 1). The institution facility can house around 60 dogs, and there were reports of CVL outbreaks on the premises approximately 3 months before the study began. The investigation was carried out from March 2019 to June 2020.
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