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1 INTRODUCTION Acute myeloid leukemia (AML) is the second-most common type of leukemia in adults and corresponds to 5% of pediatric cancer. AML is a heterogeneous malignancy, with high inter- and intra-patient heterogeneity, both at the level of genetics and immunophenotypes, posing challenges in diagnosis, classification, risk-stratification and treatment (Dohner et al., 2022; Khoury et al., 2022).
1 Introduction Polychromatic flow cytometry facilitates comprehensive cellular analysis by enabling the simultaneous detection of multiple fluorochromes on individual cells. However, due to spectral overlap, photons emitted by each fluorochrome are typically detected by multiple detectors. Consequently, the signal measured in any single detector represents a composite of emissions from several fluorochromes.
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