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Correction to: Nature Structural & Molecular Biology https://doi.org/10.1038/s41594-026-01754-2, published online 18 February 2026. In the version of the article initially published, the bottom-left label in the left panel of Fig. 5c (urea–PAGE analysis of 32P-labeled msDNA-cleavage products), was “20” but should have been “25”. This error has now been amended in the HTML and PDF versions of the article.
Abstract Retrons are prokaryotic reverse transcriptase systems that produce multicopy single-stranded DNA (msDNA), yet the principles by which they mediate antiviral defense remain largely unresolved. Here we investigate the mechanism of Escherichia coli Eco2, a minimal retron composed of a single reverse transcriptase–nuclease fusion protein.
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