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Abstract The alpha-helix is the fundamental building block of protein structure. Understanding the physical principles that determine how a specific amino acid sequence defines helicity is a key step towards elucidating the sequence-structure relationship. An established method for quantitation of helix content using circular dichroism (CD) relies on the linear spectroscopic model.
Gemma L. Gordon1, Henriette L. Capel1, Bora Guloglu1, Eve Richardson1, Ryan L. Stafford2 and Charlotte M. Deane1* 1Oxford Protein Informatics Group, Department of Statistics, University of Oxford, Oxford, United Kingdom 2Twist Bioscience, South San Francisco, CA, United States Antibodies are the largest class of biotherapeutics. However, in recent years, single-domain antibodies have gained traction due to their smaller size and comparable binding affinity.
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